THE COMPOSITION AND AMINO ACID END-GROUPS OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES
نویسندگان
چکیده
منابع مشابه
D-glyceraldehyde 3-phosphate dehydrogenases of higher plants.
The d-glyceraldehyde 3-P dehydrogenases of spinach leaf, pea seed, and pea shoot were purified. The NADP and NAD-linked enzymes of either spinach leaves and pea shoots could not be separated. Changes in the ratio of NADP- to NAD-linked activity of the spinach leaf and pea shoot enzymes were observed during both purification and storage of crude extracts. The spinach leaf, pea shoot, and pea see...
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A method is described for the isolation of glyceraldehyde 3.phosphate dehydrogenase from rabbit liver. The enzyme has been crystallized as the NAD complex and its chemical and physical properties have been compared with those of the muscle enzyme. The kinetics of the reversible reaction catalyzed by the dehydrogenases is sensitive to temperature and ionic strength and has been examined at 37, p...
متن کاملAmino acid control of the human glyceraldehyde 3-phosphate dehydrogenase gene transcription in hepatocyte.
Glutamine (Gln) is the most potent of the amino acids (AAs) that regulate liver anabolism, and its effect is similar to that of insulin in peripheral tissues. However, the influence of AAs on regulation of metabolic enzyme-encoding genes is not known at the molecular level in liver. We now report that Gln and some essential AAs activate the human GAPDH gene that codes for GAPDH, a central enzym...
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The tryptic peptide containing the active-site cysteine in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus 1503 was isolated after inhibition of the enzyme with (14)C-iodoacetate. The amino acid sequence of the 20-residue peptide was determined by 19 successive cycles of dansyl-Edman degradation. The sequence shows considerable homology with its counterparts from mesop...
متن کاملGlyceraldehyde 3-phosphate dehydrogenase: Amino acid sequence of enzyme from baker's yeast.
Glyceraldehyde 3-phosphate dehydrogenase* (GPDH) from the skeletal muscle of a wide range of organisms possesses very similar enzymic and molecular properties [ 1,2] . Within mammals the primary structure is very highly conserved [3] and the amino acid sequence around the essential thiol group is identical in all the species that have been studied [3,4] . More striking still is the considerable...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1953
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52327-4